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Image Search Results
Journal: Frontiers in Plant Science
Article Title: Proteasome Activity Profiling Uncovers Alteration of Catalytic β2 and β5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots
doi: 10.3389/fpls.2017.00107
Figure Lengend Snippet: Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or deglycosylation mix. (D) Enzymatic deglycosylation of Bovine Fetuin was used as a positive control.
Article Snippet: The denatured proteins were chilled on ice for 5 min. To the denatured samples 5 μl of
Techniques: Activity Assay, Labeling, De-Phosphorylation Assay, Positive Control